Faster Total Nucleic Acid Isolation From FFPE Tissues - Biotechnology and Molecular Medicine

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Faster Total Nucleic Acid Isolation From FFPE Tissues

Author:	thaya
Dated:	Wednesday, October 08 2008 @ 08:04 AM CDT
Viewed:	23,605 times

UPDATE
Faster Total Nucleic Acid Isolation From FFPE Tissues
Ambion® RecoverAll™ Total Nucleic Acid Isolation Kit

The Ambion RecoverAll Total Nucleic Acid Isolation Kit efficiently isolates all nucleic acids, including miRNA, from formalin- or paraformalin-fixed, paraffin-embedded (FFPE) tissues. Up to four 20 µm sections, or up to 35 mg of unsectioned co re samples, can be processed per reaction. Recent improvements to the kit have reduced hands-on time and simplified the workflow, so that the isolation of nucleic acids from FFPE tissue is 67% faster and simpler than ever.

• RNA isolation time is reduced to 90 min
(30 min digestion + 1 hour processing time) from 4.5 hours (3 hour digestion + 1.5 hour processing time)

• DNA isolation time is reduced to 17 hours
(overnight digestion + 1 hour processing time) from 3 days (48 hour digestion + 2 hour processing time)

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